Anti-Smad9 Antibody Picoband™

Product Name

Anti-Smad9 Antibody Picoband™

View all Smad9 Antibodies

SKU/Catalog Number

A04932-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Smad9 Antibody Picoband™ catalog # A07303-1. Tested in WB, ELISA applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Smad9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04932-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived mouse Smad9 recombinant protein (Position: Q153-H235). Mouse Smad9 shares 59.2% and 96.4% amino acid (aa) sequence identity with human and rat Smad9, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A04932-1 is reactive to SMAD9 in Mouse, Rat

Applications

A04932-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

52.493kDa

Background of Smad9

Mothers against decapentaplegic homolog 9 also known as SMAD9, SMAD8, and MADH6 is a protein that in humans is encoded by the SMAD9 gene. This gene encodes a member of a family of proteins that act as downstream effectors of the bone morphogenetic protein (BMP) signaling pathway. The encoded protein is phosphorylated by BMP receptors, which stimulates its binding to SMAD4 and translocation into the nucleus, where it functions as a regulator of transcription. Activity of this protein is important for embryonic development. Mutation of this gene results in defects in pulmonary vasculature.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Smad9 using anti-Smad9 antibody (A04932-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Smad9 antigen affinity purified polyclonal antibody (Catalog # A04932-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Smad9 at approximately 55 kDa. The expected band size for Smad9 is at 49 kDa.

Gene/Protein Information For SMAD9 (Source: Uniprot.org, NCBI)

Alternative Names

MAD homolog 9; MAD, mothers against decapentaplegic homolog 9 (Drosophila); Madh6; MADH6mothers against decapentaplegic homolog 9; MADH9; MADH9Mothers against decapentaplegic, drosophila, homolog of, 9; Mothers against DPP homolog 9; SMAD 9; SMAD family member 9SMAD8B; SMAD, mothers against DPP homolog 9 (Drosophila); SMAD, mothers against DPP homolog 9; SMAD8; SMAD8A; Smad9 SMAD9 MADH6, MADH9, PPH2, SMAD8, SMAD8/9, SMAD8A, SMAD8B SMAD family member 9 mothers against decapentaplegic homolog 9|MAD homolog 9|Mothers against decapentaplegic, drosophila, homolog of, 9|SMAD, mothers against DPP homolog 9

For more info on SMAD9, check out the SMAD9 Infographic

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In this infographic, you will see the following information for SMAD9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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